Novorec plus one step pcr cloning kit
WebNovoRec®PCR一步定向克隆试剂盒 (无缝克隆) NovoRec® PCR一步定向克隆试剂盒 (无缝克隆)是近岸蛋白质科技最新研发的专利产品。. 该 产品可以不依赖于连接酶，不受载体和目的片段的酶切位点限制，而直接用同源重组的方 法，完成目的片段与载体相连接的基因克隆. WebNovoprotein novorec pcr one step directed cloning kit Novorec Pcr One Step Directed Cloning Kit, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and …
Novorec plus one step pcr cloning kit
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WebPCR Mix 恒温扩增及链置换酶类 品牌. 其他; NovoRec® plus One step PCR Cloning Kit. 品牌：近岸蛋白(Novoprotein) 询价. 询价 ... Web26 okt. 2024 · 2.2 Plasmids and cloning. To clone the porcine BCL2 gene, total RNA was extracted by Trizol (Invitrogen) and was used for reverse transcription (Thermo Fisher). The porcine BCL2 gene was PCR-amplified from cDNA and subcloned into an in-house PCDH-EF1-GFP-T2A-MCS vector using NovoRec ® plus One step PCR
Web丁香通为您找到100条dna扩增后有杂带厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关dna扩增后有杂带产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务，诚信保障等服务，批发采购dna扩增后有杂带，上丁香通。 Web17 aug. 2024 · Using the pMD-18T plasmid containing the BpFae gene as the template, the BpFae gene (1722 bp) with different homologous sequences was amplified by PCR with primers A1/A2, D1/D2 and X1/X2 (Table 1), and the PCR products were cloned into the expression vectors pET-28a, pCold-TF and pGEX-4T-1, respectively, using the …
Web(Medtr7g027180) promoter using Gibson Assembly (NovoRec plus One step PCR Cloning Kit, Novoprotein, Shanghai, China). This vector was renamed as pKNF401.The gRNA vector pG- DT1T2.was synthesized containing the gRNA-Scaffold, AtU6-26t, transcriptional blocker sequence (25), and AtU6-26pro(synthesized by GenScript). Web29 mrt. 2024 · Then the amplified pegRNA-scaffold-fragment was cloned into the BsaI and EcoRI linearized pGL3-U6-sgRNA-PGK-puromycin with NovoRec® plus One step PCR Cloning Kit (NR005, Novoprotein) to generate ...
Web产品介绍. 本品含有Topoisomerase、含有自杀基因ccdB的载体，配合优化Buffer，能实现室温5 min完成高效连接。. 含有的自杀基因，当片段与载体连接会破坏ccdB的表达使得细胞能正常生长，反之则不能生长，实现零背 …
Web18 mei 2024 · QIAGEN PCR Cloning Kit (40) Cat. No. / ID: 231124. For 40 reactions: 2x Ligation Master Mix (200 µl), pDrive Cloning Vector (2.0 µg), Distilled water (1.7 ml) $525.00. Log in to see your account pricing. Quantity. Add to cart. QIAGEN PCR Cloning Kits are intended for molecular biology applications. moss green auto paintWeb29 mrt. 2024 · Then the amplified pegRNA-scaffold-fragment was cloned into the BsaI and EcoRI linearized pGL3-U6-sgRNA-PGK-puromycin with NovoRec® plus One step PCR Cloning Kit (NR005, Novoprotein) to generate the vector pGL3-U6-pegRNA-PGK-puromycin for the expression of pegRNA. moss greencarrierWebPrice from $9.99 to $1999.99 novorec pcr one step directed cloning kit - by Bioz Stars , 2024-01 86 / 100 stars Images novorec pcr one step directed cloning kit ( Novoprotein … moss green carhartt pantsWeb商品名称 : NR005-01A NovoRec® plus One step PCR Cloning Kit /正品代理 货 号： NR005-01A 20次 品 牌： Novoprotein 近岸蛋白 经销 货 期： 3天 规 格： 数量 + - 加入收藏 取消收藏 点赞 北京冬歌博业生物科技有限公司 联系人： 登录可见 联系电话： 登录可见 商品详情 是否为危控品：... minesweeper online githubWeb20 sep. 2024 · The plasmid backbones used in this study were derived from pCDH-CMV-MCS-EF1-puromycin and pCDH-U6-MCS-EF1-puromycin. The following expressing constructs ( Table 1) were generated using NovoRec plus One-step PCR Cloning Kit (Novoprotein, China). TABLE 1 Table 1. The DNA constructs used in this experiment. … minesweeper online team tournamenthttp://www.bioon.com.cn/reagent/show_product.asp?id=2213676 moss green cargos redditWeb反向PCR扩增制备线性化载体时，推荐使用高保真聚合酶Phanta® Max Super-Fidelity DNA Polymerase (Vazyme #P505)进行载体扩增，以减少扩增突变的引入。50 μl的PCR反应体 系中，推荐使用0.1 - 1 ng环状质粒模板，或使用预线性化质粒作为模板，以减少环状质粒 minesweeper on windows 10